Customer Service 800-243-3232

Cart Favorites

Microscopes

Microscopes  Upright Brightfield Microscope B-290 TB from OPTIKA

Microscopes are instruments used to see objects that are too small to see with the naked eye. Microscopes are used to investigate small objects and structures and have been used since the very early days of science, particularly in the fields of biology and microbiology. Microscopes come in a broad spectrum of observation methods that are useful for a wide range of applications, and that range of different observation methods is available here. Observation methods for Microscopes include Brightfield, Darkfield, Fluorescence, Polarized Light, Phase Contrast and Material Science Metallography (MET). Each of these is best suited to specific applications.

Brightfield Microscopes

Brightfield microscopy is the most elementary form of microscope illumination techniques, generally used in life sciences, microbiology and bacteriology. Its name is derived from the fact that the specimen is dark and contrasted by the surrounding bright viewing field. Since many organic specimens are transparent or opaque, staining is required to cause the contrast that allows them to be visible under the microscope.

Darkfield Microscopes

Darkfield microscopes are ideal for viewing objects like algae, plankton, insects, fibers, hairs, yeast, crystals, thin polymers and some ceramics that are unstained, transparent and absorb little or no light. They are useful in examining external details (outlines, edges) and surface defects than internal structure. Darkfield is being replaced by the phase contrast method which provides more accurate and contrasted images.

Fluorescence Microscopes Inverted Florescence Microscope with Petri Dish from OPTIKA

The specimen is illuminated with light of specific wavelengths which are absorbed by the fluorophores, causing them to emit light of a certain color. The filters and the dichroic mirror are chosen to match the spectral excitation and emission characteristics of the fluorophore used to label the specimen. Fluorescence microscopy requires very intense illumination (like HBO and LED) that traditional light sources cannot provide.

Polarized Light Microscopes

Polarized light microscopy is best known for geological applications, such as the study of minerals in rock thin sections, and can be used to perform investigations on many other materials. What is needed for a polarizing microscope is a polarizer and analyzer filters, Bertrand lens for conoscopic observation, compensator plates, and high-precision rotatable stages.

Phase Contrast Microscopes

Phase contrast microscopy is best known to produce high-contrast images of transparent specimens, such as living cells (usually in culture), microorganisms, thin tissue slices, fibers, latex dispersions and glass fragments with no need for staining. Phase contrast has generally replaced 95% of the darkfield, being more convenient and comfortable. Microscope Objectives from OPTIKA

Material Science/Metallography (MET) Microscopes 

Metallographic is reflected light microscopy, a method for imaging specimens that remain opaque even when ground to a thickness of few microns. The range of specimens includes most metals, ores, ceramics, many polymers, semiconductors (unprocessed silicon, wafers, and integrated circuits), coal, plastics, paint, paper, wood, leather, glass inclusions, and a wide variety of specialized materials.


 

Save up to 25%!
Digital Brightfield Microscope | B-290TB Upright Microscope by OPTIKA

Digital Brightfield Microscope | B-290TB from OPTIKA


The latest OPTIKA digital microscope with Windows tablet PC and integrated camera combines high-end optics with innovative digital technology for microscopic imaging.
compare
reviews manuals
Inverted Microscope| IM-3 Series Microscopes from OPTIKA

Inverted Microscope| IM-3 Series Microscopes from OPTIKA


IM-3 Series Microscopes from OPTIKA features options for Phase Contrast Microscopy, Fluorescence Microscopy & Material Science (MET) Microscopy
compare
reviews manuals

     
  •  

Applications for Microscopes:  

Education Industry
• Life Science • Metals
• Clinic/Diagnostic – Human and Animal • Plastics and Polymers
• Research • Glass and Ceramic
• Botanic • Rubber Compounds
• Homeopathy • Automotive
Fine Arts • Marine and Naval
• Restoration • Aerospace
• Museum • Paintings
Earth Science • Electronics and Semiconductors
• Geology • Wood
• Mineralogy • Stonesetting
• Petrology • Gems and Diamonds
Environmental • Textiles
• Water • Oil and Gas
• Asbestos • Pharmaceutical
• Air • Food
Forensics • Paper